How are my miRVEL data analyzed with Kangooroo?

The miRVEL Discovery and Profiling data analysis pipelines include:

• FASTQC analysis of raw FASTQ files (fastqc V012.1)

• Adapter Trimming (fastp V0.23.4)

• UMI deduplication (umicollapse-1.0.0 only for miRVEL Discovery Data)

• UMI Extraction (UMI-tools V1.1.6, only for miRVEL Discovery Data)

• Alignment (Bowtie V1.3.1)

• Mirtop Quantification (seqcluster V1.2.9 and mirtop V0.4.28)

• Genome quantification (Bowtie V1.3.1 and Samtools V1.21)

• Differential gene expression analysis (DESeq2 V1.18.1)