What does a typical library trace of miRVEL Profiling Small RNA-Seq Library look like?

Peaks at 165 bp correspond to the miRNA library.

A smaller peak at 143 bp indicates linker-linker artifacts which can be removed with an additional clean-up of the lane mix with magnetic beads as described in Appendix G of the miRVEL Profiling Small RNA-Seq Library Prep Kit User Guide.

Figure | Bioanalyzer traces of miRVEL Profiling Small RNA-Seq libraries synthesized from 1000 ng (red trace, 14 cycles, 1x A3 and 1x A5) and 100 ng Human Brain Total RNA (HBTR) (blue trace, 15 cycles, 0.5x A3 and 0.5x A5), as well as 6 μl of TraPR purified small RNA from Arabidopsis thaliana leaves (green trace, 17 cycles, 0.25x A3 and 0.25x A5). 1 μl of each library was loaded onto a High Sensitivity DNA chip.