What output files do I get for miRVEL data analysis with Kangooroo?
The result files included in the bundle-results folder available for download are:
Trimmed fastqc report for each sample
Counting folder for each sample including three output files for both mature and mature_hairpin miRNA (i.e., in total six output files):
o « fractional » :This file contains all the reads mapped to the genome with the number of reads being divided by the number of time these reads are aligned to the genome.
o « multimap »: This file contains all the reads mapped to the genome including multimappers.
o « unique »: This file contains only the uniquely mapped reads to the genome and is typically used as input for DESeq 2 analysis.
Counting_summary folder similar to the counting folder but which regroups data from all samples.
For differential expression analysis: normalized counts.tsv, pairwise summary.txt, results .tsv files and MA plots available for mature and hairpin miRNAs for each pairwise comparison (when differential gene expression is specified).
NOTE: In the DE analysis tab on the Kangooroo platform, Volcano, MA and PCA plots for fractional counts are displayed and available for download.
PDF report summarizing all the results
NOTE: Aligned.bam files are not part of the bundle-result folder but can be downloaded separately. Please be mindful that depending on the size and number of files to download, you may need to purchase additional quotas.
If additional quotas are required, please contact sales@lexogen.com.