Are the data obtained with QuantSeq with UDI V1 and V2 comparable?

Yes. We observe a high correlation between data obtained with the QuantSeq 3‘ mRNA-Seq V2 Library Prep Kit FWD with Unique Dual Indices (12nt) and the QuantSeq 3‘ mRNA-Seq Library Prep Kit FWD with Unique Dual Indices (V1).

Figure | Correlation plot obtained with 500ng of UHR + SIRV3 control Set, comparing QuantSeq UDI “V1” (x axis) and the new “V2” (y axis).