How can I analyze my QuantSeq with UDI V2 data?
Our QuantSeq FWD V2 kit comes with a voucher code to access free data analysis on our web-based platform Kangooroo.
Kangooroo offers tailored data analysis pipelines optimized for QuantSeq FWD data with or without UMIs!
For more information, please visit our website Lexogen - Kangooroo and our online Kangooroo Data AnalysisFAQs!
If additional voucher codes are needed, please contact us at sales@lexogen.com.
NOTE: Our QuantSeq FWD pipelines utilize single-read data only. Please, only upload Read 1 FASTQ files.
If you prefer to analyze your QuantSeq FWD data on your own, as reference you can find below the general workflow with key steps to successfully analysis your QuantSeq data!
Key steps of the general workflow to use to analyze QuantSeq FWD data:
QuantSeq FWD | QuantSeq FWD - UMI |
|---|---|
- | UMI extraction |
Trimming | Trimming |
Mapping | Mapping |
- | UMI collapsing |
Gene Read counting | Gene Read counting |
Differential Expression Analysis | Differential Expression Analysis |