Can I get script files for QuantSeq automation?
Our QuantSeq library prep protocols have been automated on most major liquid handers (please see this FAQ).
For automation scripts, we invite you to contact the FAS of the liquid handler manufacturer to obtain the latest and most appropriate script for our protocol on your instrument. Scripts may vary based on the liquid handler used, deck setup, software versions, consumables, and manual interventions. Therefore, for setting up automation of our protocols, we prefer to direct our customers to the specific FAS of the platform used (or to be used).
NOTE: Internally, we use the Biomek i7 platform for automation. Connect us with your local FAS and we can provide the latest script file and work together to obtain the optimal program script for your particular instrument.
Please find below our general recommendations for automation settings:
As mentioned, contact the FAS of the liquid handler to check the compatibility of your instrument with the latest version of the automation script protocol.
Perform a dry run to ensure there are no errors or incompatibilities.
Perform a dummy run for volume checks after program installation. We recommend wet runs before using real samples to ensure reaction volumes are correct with the dummy reagents you already have. We recommend these be used before real samples are run to ensure reaction volumes are correct.
Purification module for automation optimization are not included in the dummy kit. We recommend the purification steps be optimized using real reagents due to the critical nature of these steps because these buffers are hard to mimic correctly.
Perform a test run for a small subset of sample using a reference RNA. It is recommend to run a manual prep in parallel to compare results downstream.
NOTE: Automation scripts are typically provided for the full library prep protocol. Automating only the post-PCR purification can also be achieved by modifying an existing Post-PCR SPRI bead purification program. Please ensure the double bind steps are included.
IMPORTANT: Lexogen is not an automation company, and although we're happy to support our customers as much as we can (e.g., looking at the results to help the troubleshooting and providing our input), any script modifications and the initial steps of installations should be performed by the platform provider.