Can I use paired-end sequencing for QuantSeq REV libraries?

Yes!

You can perform Paired-end (PE) sequencing using QuantSeq REV. Please note QuantSeq REV requires a Custom Sequencing Primer (CSP) for Read 1 sequencing in order to generate reads that begin at the exact 3' end of the transcript.

For more information on how to sequence your QuantSeq REV libraries, please see this FAQ.