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Can SLAMseq be used for analyzing nascent expression levels and kinetics of small RNAs?

Yes, SLAMseq can be used for analysis of small RNAs. The method has been used in the following publications:

  • Drosophila miRNAs: Reichholf B, Herzog VA, Fasching N, Manzenreither RA, Sowemimo I, Ameres SL. Time-Resolved Small RNA Sequencing Unravels the Molecular Principles of MicroRNA Homeostasis. Mol Cell. 2019;75(4):756-768.e7. doi:10.1016/j.molcel.2019.06.018

  • Mouse piRNAs: Sharma U, Sun F, Conine CC, et al. Small RNAs Are Trafficked from the Epididymis to Developing Mammalian Sperm. Dev Cell. 2018;46(4):481-494.e6. doi:10.1016/j.devcel.2018.06.023

For specific enquires related to small RNA SLAMseq experiments with Lexogen’s SLAMseq and Small RNA-Seq Library Prep kits, please contact

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