Does RiboCop for Plants efficiently deplete rRNA from low quality RNA?

Yes, RiboCop for Plants reduces rRNA reads of low-quality Arabidopsis thaliana RNA to ~4% when 1 µg inputs are used. When starting with 1-100 ng of low-quality RNA material, RiboCop for Plants reduces rRNA reads to ~0.4-0.7%.

Please note that depletion rates may vary depending on the quality of the RNA and purity of the sample. Contaminants, such as salts, metal ions, and organic solvents, may have a negative impact on the efficiency of the protocol.