There are several options to analyze your miRVEL data!
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Kangooroo data analysis platform
Our miRVEL kits come with a voucher code to access free data analysis on our web-based platform Kangooroo.
For more information, please visit our website Lexogen - Kangooroo and our online Kangooroo Data AnalysisFAQs!
If additional voucher codes are needed, please contact us at sales@lexogen.com.
NOTE: Our miRVEL pipelines utilize single-read data only. Please, only upload Read 1 FASTQ files.
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Service project
miRVEL small RNA data can be analyzed as a Service project with Lexogen! For more information, please visit our Bioinformatic Services webpage and contact us at services@lexogen.com.
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Independent analysis
You can analyze the data on your own. As reference, you can use the following publicly available data analysis workflows:
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Small RNA analysis pipeline built using modified Nextflow workflow tool: https://nf-co.re/smrnaseq/1.0.0.
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Workflow adapted from Tam et al., (2015) Optimization of miRNA-seq data preprocessing. Briefings in Bioinformatics 16(6). This paper also provides an additional comparison of programs for read mapping (alignment), normalization methods, and differential expression analysis.
NOTE: If you need to include UMI extraction and deduplication for miRVEL Discovery libraries, please contact support@lexogen.com.