How can I analyze my miRVEL Small RNA sequencing data ?

There are several options to analyze your miRVEL data!

You can analyze your miRVEL data (Discovery and Profiling) on our web-based platform Kangooroo.

To start the data analysis, you will need to purchase a voucher code at sales@lexogen.com.

For more information on our Kangooroo platform and miRVEL data analysis please check our Kangooroo Data Analysis FAQs!

miRVEL small RNA data can be analyzed as a Service project with Lexogen! For more information, please visit our Bioinformatic Services webpage and contact us at services@lexogen.com.

You can analyze the data on your own. As reference, you can use the following publicly available data analysis workflows:

Small RNA analysis pipeline built using modified Nextflow workflow tool: https://nf-co.re/smrnaseq/1.0.0.
Workflow adapted from Tam et al., (2015) Optimization of miRNA-seq data preprocessing. Briefings in Bioinformatics 16(6). This paper also provides an additional comparison of programs for read mapping (alignment), normalization methods, and differential expression analysis.

NOTE: If you need to include UMI extraction and deduplication for miRVEL Discovery libraries, please contact support@lexogen.com.