How do I analyze my LUTHOR HD Pool data?

LUTHOR HD Pool data can be analyzed as a Service project with Lexogen! For more information, please visit our Bioinformatic Services webpage and contact us at services@lexogen.com.

Alternatively, you can analyze the data on your own. As reference you can find below the general workflow with key steps to successfully analysis your LUTHOR HD Pool data!

NOTE: LUTHOR HD Pool pipeline utilizes paired-end data only. Please, upload both Reads (1 and 2) FASTQ files for your data analysis!

Key steps of the general workflow to use to analyze LUTHOR HD Pool data:

Following demultiplexing which requires the use of Lexogen’s idemux tool (available in C++ or Python versions), in principle, all further steps for trimming, mapping, UMI processing, and counting can be integrated into existing standard data analysis pipelines. For further questions about the pipeline, please contact support@lexogen.com.