How many samples can I process in a single run?

Depending on your deck setup, the automated protocol can be adapted for preparing up to 96 samples in parallel.
An existing protocol for the Hamilton STAR is designed to process up to 48 samples at once.

Existing protocols for the Agilent Bravo NGS Workstation (B), and Beckman Coulter Biomek FxP and i7, can process up to 96 samples in parallel.

The program for Agilent´s Bravo runs multiples of 8 reactions (i.e., column-wise from columns 1-12).

The PerkinElmer Sciclone, also runs reactions in multiples of 8 samples (column-wise). Any number of columns from 1 to 12 can be run in parallel. The machine will always take the barcodes from the first N wells of the barcode plate, starting with column 1.

The program for Perkin Elmer Zephyr is only available for the purification steps. Please use the protocol for the Perkin Elmer Sciclone for the Phase1-PrePCR automated protocol.