SIRV RNA is spiked into samples prior to RNA Extraction, or into total RNA prior to pre-processing or library preparation. The amount of SIRV RNA to add to each sample depends on:
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The total RNA input amount,
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The type of library preparation method used, and
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The intended fraction of SIRV reads to be detected in the final sequencing output.
Use our Updated SIRV Calculator Worksheets to plan your spike-in experiments!
Download the User Guides and Reference Annotations from our SIRV Download page.
Access the updated SIRV Suite Data Analysis Tools.