How to use SIRVs
SIRV RNA is spiked into samples prior to RNA Extraction, or into total RNA prior to pre-processing or library preparation. The amount of SIRV RNA to add to each sample depends on three things:
the total RNA input amount,
type of library preparation method used, and
the intended fraction of SIRV reads to be detected in the final sequencing output.
Use our Updated SIRV Calculator Worksheets to plan your spike-in experiments!
Download the User Guides and Reference Annotations from our SIRV Download page.
Access the updated SIRV Suite Data Analysis Tools.