Is QuantSeq with UDI V2 suitable for the analysis of FFPE samples?
Yes. Low-quality and FFPE samples can be used with QuantSeq with UDI V2.
When comparing data generated using QuantSeq with UDI V2 from fresh frozen (FF) and FFPE samples, we observe a high overlap of detected genes.
Figure | Sequencing data obtained with 10ng input from two different mouse spleen samples, 1M reads, 1cpm, 5cpm, and 10cpm (from left to right).
RIN values: 3.7 (FF) and 2.9 (FFPE). DV200 values: 90% (FF) and 9% (FFPE).