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What are the minimum and maximum number of samples I can pool into a single tube?

The minimum number of samples to be processed and pooled at a time is 8 i.e., one column of the dried-in i1 Primer plate. The maximum number of samples to processed and pooled at a time depends on the input. Please see the following table:

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Please use the next table as a guideline for the number of samples to be pooled following the initial THOR reaction (Steps 1 – 7). Importantly, if you intend to pool >48 samples, please purify 2x 48 reaction pools and combine during the purification at Step 11:

image-20240624-160604.png

 

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