What cycle number do I need to use to amplify my targeted sequencing library?

The required cycle number depends on multiple factors such as the number of targeted transcripts, their abundance, the primers and the primer concentrations used, and the RNA input amount and quality.

Therefore, to determine of the optimal number of PCR cycles, we strongly recommend taking advantage of the qPCR assay utilizing the PCR Add-on and Reamplification Kit (Cat. no. 208).