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What input amounts should I use?

FFPE tissue sample input should be 100-800 mm2 in surface or 1-15 mg in weight, and up to 10 µm in thickness. For higher input amounts, we strongly recommend performing a pilot experiment first to evaluate RNA extraction efficiency. If you have questions, please contact support@lexogen.com.

Below are general recommendations for maximum input, depending on the thickness and surface area of the FFPE slices:

Slice thickness

Maximum input

5 µm

1 slice with 1,600 mm2

2 slices with 800 mm2

3 slices with 530 mm2

4 slices with 400 mm2

10 µm

1 slice with 800 mm2

2 slices with 400 mm2

3 slices with 260 mm2

20 µm

1 slice with 400 mm2

2 slices with 200 mm2

3 slices with 130 mm2

4 slices with 100 mm2

For lower inputs, please refer to the following FAQ: How can I increase my final yield?

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