The presence of gDNA contamination can strongly impact the sequencing data and lead to biases and quantification issues during data analysis.
A typical signature of gDNA contamination includes:
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A high proportion of intronic and intergenic reads
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Loss of strand specificity (i.e., reads mapping to both strands)
NOTE: For more information on gDNA contamination and DNAse I treatment, please, check our RNA LEXICON Chapter #5.