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What is the typical fragment size for QuantSeq Libraries?

The QuantSeq protocols are optimized for shorter reads (SR75 – 100) and yield mean library sizes of about 335 – 456 bp, with mean insert sizes of 203 – 324 bp.

It is possible to extend the length of QuantSeq FWD libraries, using the QuantSeq-Flex First Strand Synthesis Module V2 (Cat. No. 166). See this FAQ for an overview, or contact for more information.

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