What is the typical library size distribution for QuantSeq FFPE libraries?
The library shape, size, and yield may vary depending on the sample input and quality. Heavily degraded FFPE samples typically generate shorter libraries than FFPE samples of higher quality.
Most inserts are expected to be >75 bp, corresponding to final libraries with fragment sizes >200 bp.
The figure below depicts examples of QuantSeq FFPE libraries obtained from various FFPE RNA samples differing in RNA input amounts and quality: