Processing high quality RNA from bacterial monocultures with the Gram Positive or Gram Negative Probe Mixes typically results in less than 1 % remaining rRNA reads. Depletion rates for the META Probe Mix using RNA from mixed bacterial samples can vary depending on the sample.
NOTE: Additionally, depletion rates may vary depending on the quality of the RNA and purity of the sample. Contaminants, such as salts, metal ions, and organic solvents, may have a negative impact on the efficiency of the protocol.
Figure | Ribocop rRNA Depletion for Mixed Bacterial Samples (META) efficiently removes rRNA from various bacterial samples.