Which adaptor dilution should be used for small RNA library preparation with TraPR-enriched sRNAs?

The recommended adaptor dilution depends on the small RNA library prep kit used. Please see the guidelines below:

• miRVEL Discovery kit (Cat. No. 242), use a 0.2x dilution for 3´adaptor and 25x dilution for 5´adaptor (See Appendix B of the User Guide).

• miRVEL Profiling kit (Cat. No. 243): use a 0.1x adaptor dilution (See Appendix B of the User Guide).

• Small RNA-Seq Library Prep kit (Cat. No. 052): use a 0.5x to 0.3x adaptor dilution (See Appendix B of the User Guide).

For certain sample types even higher adaptor dilutions can be beneficial. Please contact support@lexogen.com for details.