Which sequencing platforms are suitable for Small RNA-Seq libraries? How much Small RNA-Seq library should be loaded for sequencing?
The Small RNA-Seq Library Prep Kit is appropriate for sequencing on Illumina platforms (HiSeq2000, HiSeq3000, HiSeq4000, NextSeq 500 / 550, NextSeq 2000, NovaSeq 6000, MiSeq, MiniSeq, and GAIIX).
The libraries are compatible with standard Illumina sequencing primers for TruSeq Small RNA and no custom sequencing primers are required.
For current loading amount recommendations please see: Do you have recommended loading concentrations for Lexogen libraries on Illumina sequencing instruments?