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Is there a size limit for FASTQ files upload? What should I do if my FASTQ files exceed the size limit?

Is there a size limit for FASTQ files upload?

Yes.

Please find below the maximum FASTQ file size (quota) included in the voucher code for data upload:

Library prep Kit(s)

File size upload (quotas)

  • QuantSeq (FWD, FWD-UMI, REV)

  • miRVEL (Discovery and Profiling)

~ 1,5 GB / file

  • QuantSeq FFPE

  • LUTHOR HD

~ 3 GB / file

  • CORALL

  • Whole Transcriptome Sequencing

~ 10 GB / file

What should I do if my FASTQ files exceed the size limit?

If FASTQ files slightly exceed the size limit, you can attempt to compress your files with 7-zip for windows computer.

For Mac Os and Linux, you can use the following command line:

gunzip -c some_file.fastq.gz | gzip -9 > some_higher_compressed_file.fastq.gz

Alternatively, you could also try to sub-sample reads with awk. Please check this command line tutorial

 If needed, additional quotas can be purchased separately at sales@lexogen.com.

Here are the catalog numbers you will need:

Catalog No.

Product Name

240.1.5

Kangooroo data analysis - up to 1.5 GB input file

240.3

Kangooroo data analysis - up to 3 GB input file

240.6

Kangooroo data analysis - up to 6 GB input file

240.10

Kangooroo data analysis - up to 10 GB input file

240.15

Kangooroo data analysis - up to 15 GB input file

240.1G

Kangooroo New Genome Implementation for Data Analysis

240.1D

Kangooroo data download - 1GB

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