Which Sequencing Platforms are Lexogen Libraries Compatible with?

Full-length, indexed Lexogen libraries feature P5/P7 surface-binding adapter and internal sequencing primer-binding sequences that are directly compatible with all Illumina sequencing instruments and chemistries.

For adapter details please consult the “Sequencing” Appendix of the User Guide for the respective Lexogen library preparation kit.

Lexogen library compatibility with a range of popular and emerging sequencing platform technologies and instruments, is outlined below.

Disclaimer!

Library conversion compatibility for Lexogen library types is briefly outlined where relevant. This information serves as a guideline only and is subject to change.

Lexogen is not responsible for the performance of library conversion protocols.

Library sequencing compatibility or conversion requirements outlined below may vary according to instrument, sequencing chemistry, or software version updates, or specific project requirements. Please contact the sequencing platform manufacturer for specific support regarding, library conversion protocol and product requirements, protocol performance outcomes, and sequencing run reagent and setting details.

Lexogen provides support only to ensure that libraries produced using our kits and protocols meet input requirements for sequencing provider workflows (e.g. input amounts, and input material type). Lexogen Technical Support is also happy to help you consult with sequencing provider support teams in order to verify compatibility for new conversion approaches. Lexogen’s support is based only on the verified experience of Lexogen, feedback from Lexogen customers, or method details from peer-reviewed publications.

Short-read platforms with direct compatibility

The following platforms support direct sequencing of the following library types: QuantSeq (FWD / FFPE / REV / -Pool and combined SLAMse); CORALL (RTM / RTL / FFPE); Luthor (HD / HD-Pool); miRVEL (Discovery / Profiling), and Small RNA-Seq library types.

	Illumina	Element Biosciences
Instruments	iSeq i100 MiniSeq* MiSeq /MiSeqi100 NextSeq 500/550/1000/2000 NovaSeq 6000/X/X+	AVITI AVITI LT AVITI24
Chemistries	SBS Standard, X-LEAP	CloudBreak Freestyle only

*discontinued but supported by Illumina for existing customers until 2029.

Short-read platforms that require library conversion

The following platforms support sequencing after library conversion, for the following library types: QuantSeq (FWD / FFPE / REV / -Pool and combined with SLAMseq); CORALL (RTM / RTL / FFPE); Luthor (HD / HD-Pool); miRVEL (Discovery / Profiling), and Small RNA-Seq library types.

NOTE: Some conversion methods can also be performed on pools of indexed libraries (i.e. lanemixes).

MGI (DNBSEQ)

🤝 Lexogen Partner

🏅 Verified by Lexogen

Element Biosciences

🏅Verified by Lexogen

Singular Genomics

🏅Verified by Lexogen

Ultima Genomics

Pacific Biosciences

Instruments:

BGISEQ-500RS, MGISEQ-200RS, MGISEQ-2000RS, DNBSEQ-G50RS , DNBSEQ-G400RS, (TBC: DNBSEQ-G99, DNBSEQ-T-series)

AVITI, AVITI LT, AVITI 24

G4

UG100

Onso

Method

Amplified full-length, indexed dsDNA libraries (or pools of libraries) are circularized to produce circular ssDNA library fragments.

Amplified full-length, indexed dsDNA libraries (or pools of libraries) have terminal Element-Biosciences adapters added, either through; circularization or PCR

Amplified full-length, indexed dsDNA libraries are subject to PCR to add G4-compatible flow cell-binding adapters (and indexes). Conversion cannot be performed on pools of indexed libraries (i.e. lanemixes).

Non-amplified cDNA, or amplified, full-length indexed dsDNA libraries are subject to PCR to add UG100-compatible adapters (with or without indexes).

Amplified full-length, indexed dsDNA libraries are subject to PCR to add PacBio compatible adapter sequences. Conversion is not recommended for pools of indexed libraries (i.e. lanemixes).

Support contact

MGI-service@mgi-tech.com

support@elembio.io

care@singulargenomics.com

support@ultimagen.com

support@pacb.com

Long-read sequencing platforms

Long-Read Sequencing Platform technologies are compatible with Lexogen’s TeloPrime Full-Length cDNA Amplification Kit (Cat. No. 013).

IMPORTANT: Conversion requirements for new sequencing instruments, and/or new vs legacy versions of kit chemistry, should be confirmed with the instrument manufacturer directly.

	Pacific Biosciences 🏅 Verified by Lexogen Customers	Oxford Nanopore Technologies 🏅 Verified by Lexogen Customers
Instruments:	Sequel I/II*, Revio, Vega	MinION, GridION
Support contacts	support@pacb.com - confirm kit & protocols, barcode and adapter sequence requirements	support@nanoporetech.com or https://nanoporetech.com/support/customer-support (live chat) -confirm kit requirements and protocol details.
Methods	Custom forward and reverse primers featuring 5' overhangs that encode PacBio or ONT adapter sequences are required. These custom primers replace the FP and RP PCR primers for TeloPrime cDNA Amplification. Using custom primers with adapter sequences during TeloPrime reverse transcription is also possible. The amplified TeloPrime cDNA can then be used for downstream PCR or adapter-ligation based library construction. To confirm the specific method and kit requirements please consult with PacBio or ONT technical support.
Support Resources	support@lexogen.com - Lexogen can provide support to confirm custom primer design compatibility with TeloPrime and optimization of cDNA amplification. Lexogen’s experience with TeloPrime conversion for PacBio sequencing refers to Sequel I and II platforms, and the PCR Rapid Barcoding Kit (now discontinued) with MinION, for Oxford Nanopore sequencing. For additional information see: FAQs https://faqs.lexogen.com/faq/how-can-i-use-teloprime-to-prepare-full-length-cdn https://faqs.lexogen.com/faq/should-i-perform-size-selection-of-teloprime-v2-cd https://faqs.lexogen.com/faq/can-i-use-sample-barcodes-for-multiplexing-telopri Key Publications Note: these publications may reference older versions of library conversion kit chemistry and/or instrument types https://www.lexogen.com/publications/teloprime-publications/ https://www.nature.com/articles/s41467-024-49523-3#Sec9 https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1012820#sec011 https://peercommunityjournal.org/item/10_24072_pcjournal_457/ https://www.mdpi.com/2073-4409/13/5/383 https://www.cell.com/cell-genomics/fulltext/S2666-979X(25)00109-0

Non-compatible technologies

Instruments not listed in this FAQ either do not support Lexogen library sequencing or lack sufficient information to determine compatibility. For inquiries about other instruments, please contact support@lexogen.com.

Company	Instrument(s)	Reason
Thermo Fisher Scientific	Ion Torrent (Genexus; Ion Chef; GeneStudio, Ion PGM)	Adapters must be added during library generation. Adapter primers not available from Lexogen.
Roche	454; SBX	454 is discontinued. SBX is in development (no information available)
Salus BioMed	Nimbo, Pro, EVO	Insufficient information to determine compatibility.
10X	Chromium, Chromium iX/X, Xenium, Visium	These microfluidics and spatial transcriptomics instruments do not perform in-solution sequencing.
Singular Genomics	GX4	Spatial transcriptomics instrument, does not perform in-solution sequencing.