Can I analyze my MGI data on Kangooroo?
Yes!
Lexogen libraries sequenced on MGI sequencing instruments can be analyzed on Kangooroo.
For libraries including UMIs such as QuantSeq-FWD with UMIs, QuantSeq FFPE, CORALL and LUTHOR HD, the read header of MGI FASTQ files must be first converted to Illumina format. To do so, you can use our MGI read converter tool available on our Github webpage.
Figure 1: Conversion of MGI read header to Illumina read header format*.
For any other Lexogen libraries (without UMIs), you can directly upload your MGI FASTQ files on the Kangooroo platform!
*Source https://sagc-bioinformatics.github.io/mgikit/demultiplex.