Do I need special de-multiplexing software tools for this product?

Demultiplexing can be carried out by standard Illumina tools (BaseSpace Sequence Hub and bcl2fastq2). Index sequences (UDI12A_0001-0384 and UDI12B_0001-0096) are available for download at www.lexogen.com/docs/indexing.

Additionally to the standard error-correction included in the standard Illumina tools, Lexogen’s idemuxCPP tool can be used to demultiplex and/or error correction. This command line tool is available free of charge at https://github.com/Lexogen-Tools/idemuxcpp.

 Lexogen’s idemuxCPP can be used for:

• i1 barcode demultiplexing of QuantSeq-Pool libraries

• i5 and i7 demultiplexing of QuantSeq, QuantSeq-Pool, CORALL, and LUTHOR libraries

• AND Error correction of above mentioned demultiplexed reads

Please contact support@lexogen.com for more information.