Do I need to get all 4 modules or can I just get the kinetics kit(s)?

No.

If S4U labeling concentrations and incorporation rates for the defined experimental conditions are already established, you only need the SLAMseq Kinetics Kit – Anabolic Kinetics Module (Cat. No. 061), or – Catabolic Kinetics Module (Cat. No. 062) for conducting labeling experiments.

Guidelines for tested cell lines are provided in Do you have guidelines for the optimal S4U concentrations to use for different cell lines? and in the SLAMseq Kit User Guide Appendix E (p.28).

When performing the experiment for the first time, working with a new cell line and / or establishing new experimental set-ups, we highly recommend assessing cell viability and optimal S4U labeling concentrations. For this purpose, we recommend the SLAMseq Explorer Kit – Cell Viability Titration Module (Cat. No. 059).

Analyzing S4U incorporation rates with the SLAMseq Explorer Kit – S4U Incorporation Module (Cat. No. 060) also provides a way to directly measure S4U uptake in newly synthesized RNA for calculating the efficiency of S4U labeling and conversion via HPLC analysis.

NOTE: This can also be carried out via shallow sequencing of QuantSeq FWD libraries. In this case, the SLAMseq Explorer Kit – S4U Incorporation Module is not required.