Skip to main content
Skip table of contents

How are the 5’ and 3’ ends tagged?

Tagging on the 5' end is based on Lexogen´s proprietary Cap Dependent Linker Ligation (CDLL) technology. After reverse transcription and generation of a double-stranded RNA : cDNA hybrid, an adapter with a 5' C overhang facilitates atypical base-pairing with the inverted G of the cap structure. Ligation takes place using a double-strand specific ligase, by ligating the 3' end of the double-stranded Cap linker to the 3' end of the cDNA.
Tagging of the 3' end of the mRNA takes place during the reverse transcription through the use of oligo(dT) priming.

JavaScript errors detected

Please note, these errors can depend on your browser setup.

If this problem persists, please contact our support.