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How can I determine the amount of globin mapping reads in my sequencing data?

The percentage of reads mapping to globin mRNAs is calculated as the fraction of read counts uniquely mapping to globin genes, divided by the total number of uniquely mapped reads.

Uniquely mapped read counts are generated from the QuantSeq data analysis and can be downloaded among the resulting output files. Additional information related to the output files provided with the our Kangooroo QuantSeq data analysis can be found here: What output files do I get with Kangooroo?

Lists of Ensembl gene IDs for human and pig globin genes are provided in the tables below.

For further information on data analysis for QuantSeq libraries prepared with Globin Block Modules please contact support@lexogen.com.

Human Globin mRNA

Gene Symbol

Ensembl Gene ID

HBQ1

ENSG00000086506

HBZ

ENSG00000130656

HBA2

ENSG00000188536

HBG2

ENSG00000196565

HBA1

ENSG00000206172

HBM

ENSG00000206177

HBZP1

ENSG00000206178

HBE1

ENSG00000213931

HBG1

ENSG00000213934

HBD

ENSG00000223609

HBBP1

ENSG00000229988

HBB

ENSG00000244734

Pig (Sus scrofa) Globin mRNA

Gene Symbol

Ensembl Gene ID

HBZ

ENSSSCG00000007975

HBM

ENSSSCG00000007977

HBE1

ENSSSCG00000014726

HBB

ENSSSCG00000014725

HBA

ENSSSCG00000007978

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