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How can I multiplex more than 384 samples on an Illumina NovaSeq instrument if you only offer 384 different UDIs?

The NovaSeq Xp workflow enables individual lane loading on the same flow cell. Hence the multiplexing capacity can be expanded 2 times using the NovaSeq XP 2-Lane Kit or 4 times with the NovaSeq XP 4-Lane Kit. Hence, it is possible load up to 4 physically separated 384-plex UDI-indexed libraries per flow cell. For more information about the Xp Workflow, please refer to the NovaSeq™ Xp workflow Technical Note, The NovaSeq™ Xp workflow explanatory video, or contact Illumina Support.

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