How long does the generation of full-length double-stranded DNA take?

The full-length cDNA synthesis takes around 5 hours in total, including just 60 minutes of hands-on time. Endpoint PCR for amplification of TeloPrime cDNA using the V2 kits takes between 5 – 8 hours (depending on the number of PCR cycles) with only 25 minutes of hands-on time. The qPCR, which is strongly recommended, is performed prior to endpoint PCR, takes ~14 hours, and is best run overnight.