How many PCR cycles do I need for brain libraries made using the BC1 Block Module?

Typically, libraries prepared from mouse brain total RNA using BC1 Block require one additional PCR cycle compared to libraries made with the standard QuantSeq Kit reagents.

However, we strongly recommend performing a qPCR assay with The PCR Add-on and Reamplification Kit V2 (Cat. No. 208) to determine the optimal number of PCR cycles for the library amplification. For more information on the qPCR assay, please see the following FAQs:

What do I need for the qPCR Assay?

How do I calculate endpoint cycle numbers?