How many PCR cycles do I need to use for blood RNA libraries prepared using Globin Block?
Typically, libraries prepared from blood total RNA using Globin Block require one additional PCR cycle compared to libraries made with the standard QuantSeq Kit reagents.
However, we strongly recommend performing a qPCR assay with The PCR Add-on and Reamplification Kit V2 (Cat. No. 208) to determine the optimal number of PCR cycles for the library amplification. For more information on the qPCR assay, please see the following FAQs: