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How should I remove contaminating gDNA from my samples before library prep?

Since FFPE samples are prone to gDNA carry-over, it is recommended to perform a DNase treatment prior to library preparation.

When using CORALL FFPE, there are two options for DNase treating FFPE samples:

  • DNA Removal Add-on Module (Cat. No. 235) - instructions for utilizing this module in the CORALL FFPE workflow are provided in our User Guide (p. 9). Following DNA Removal, the final volume can be directly input into RiboCop.

  • SPLIT One-Step FFPE RNA Extraction Kit (Cat. No. 236 - available soon) - a 15 minute DNase treatment is seamlessly included during RNA Extraction.

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