Is there an index balance checker tool for 12 nt UDIs?

Currently not, however, using the UDIs in numerical order as the number of libraries to multiplex increases, or column-wise for increasing multiples of 8 samples, will result in optimal nucleotide balance.

For smaller numbers of samples we can also suggest the following:

• Four libraries: Use UDI12A / B_0001 - 0004 as these contain almost perfect nucleotide balance at each position of the index read

• Eight libraries: Use column 1 of the Lexogen UDI 12 nt Set A or B (UDI12A / B_0001 - 0008)

NOTE: Individual libraries within a lane or run should always be pooled at an equimolar ratio to preserve perfect nucleotide balance at each position of the index read.