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Module Overview

The table below lists the available Add-on Modules and Kits and their compatibility with Lexogen Library Prep kits.

For Lexogen’s 12 nt Unique Dual Indexing Add-on Kits and Sets, please refer to: 12 nt Unique Dual Indexing Solutions.

NOTE: The UMI and Globin or BC1 Block Modules for QuantSeq can be combined in the same library prep.

Module

Compatibility Details

PCR Add-on and Reamplification Kit V2 (Cat. No. 208)

qPCR assay for the following kits:

  • QuantSeq V2 with UDI

  • QuantSeq-Pool

  • CORALL V2 with UDI

  • LUTHOR HD

Reamplification of libraries prepared with the following kits:

  • All CORALL library prep kits (all versions)

  • All QuantSeq library prep kits (all versions)

  • QuantSeq-Pool

  • All LUTHOR library prep kits (all versions)

  • Small RNA-Seq library prep

This kit is NOT compatible with TeloPrime.

This kit is NOT recommended for qPCR assays with older versions of QuantSeq (V1) and CORALL (V1).

UMI Second Strand Synthesis Module for QuantSeq FWD (Cat. No. 081)

Kits: QuantSeq FWD Kits only (Cat. No. 191 - 196).

Sample type: all inputs, but best used for low input and FFPE sample.

This module can be used in QuantSeq libraries prepared using Globin Block Modules for QuantSeq.

NOT Compatible with: QuantSeq REV or LUTHOR.

Globin Block Modules for QuantSeq (Cat. No.s 070, 071)

Kits: QuantSeq FWD and REV kits only (Cat. No.191 - 196, 225).

Sample types: whole blood total RNA from human or pig (Sus scrofa).

This module can be used in QuantSeq libraries prepared using the UMI Second Strand Synthesis Module for QuantSeq.

NOT Compatible with: QuantSeq-Pool, CORALL, or LUTHOR.

BC1 Block Module for QuantSeq (Cat. No. 167)

Kits: QuantSeq FWD and REV kits only (Cat. No.191 - 196, 225).

Sample type: mouse brain/neuronal cell input RNA.

NOT Compatible with: QuantSeq-Pool, CORALL, or LUTHOR.

Purification Module with Magnetic Beads (Cat. No. 022)

Purification protocols included in the following kits:

  • QuantSeq FWD/FWD-UMI/REV/-Flex

  • CORALL

  • QuantSeq-Pool

  • Small RNA-Seq (size selection)

  • LUTHOR HD

  • TeloPrime (post-PCR purification only! - please enquire via support@lexogen.com for details).

Gel Extraction Module (Cat. No. 054)

Kit: Small RNA-Seq Library Prep Kit.

TeloPrime PCR Add-on Kit V2 (Cat. No. 018)

Kit: TeloPrime Full-Length cDNA Amplification Kit V2.

Protocols: qPCR assay, endpoint PCR.

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