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Modules and Add-on Kits

This section gathers Frequently Asked Questions (FAQs) related to Lexogen Modules and Add-on Kits.

The table below lists the available Add-on Modules and Kits and their compatibility with Lexogen Library Prep kits.

For Lexogen’s 12 nt Unique Dual Indexing Add-on Kits and Sets, please refer to: 12 nt Unique Dual Indexing Solutions.

NOTE: The UMI and Globin or BC1 Block Modules for QuantSeq can be combined in the same library prep.

 

Module

Compatibility Details

PCR Add-on and Reamplification Kit V2 (Cat. No. 208)

qPCR assay for the following kits:

  • QuantSeq V2 (FWD, REV and FFPE) with UDI

  • QuantSeq-Pool

  • CORALL V2 with UDI

  • LUTHOR HD

  • LUTHOR HD Pool

Reamplification of libraries prepared with the following kits:

  • All CORALL library prep kits

  • All QuantSeq library prep kits

  • All LUTHOR library prep kits

  • Small RNA-Seq library prep

This kit is NOT compatible with TeloPrime.

UMI Second Strand Synthesis Module for QuantSeq FWD (Cat. No. 081)

Kits: QuantSeq FWD Kits only (Cat. No. 191 - 196).

Sample type: all inputs, but best used for low input and FFPE samples.

This module can be used in QuantSeq libraries prepared using Globin Block Modules for QuantSeq.

NOT Compatible with: QuantSeq REV, FFPE and Pool, CORALL, LUTHOR HD and LUTHOR HD Pool.

Globin Block Modules for QuantSeq (Cat. No.s 070, 071)

Kits: QuantSeq FWD and REV kits only (Cat. No.191 - 196, 225).

Sample types: whole blood total RNA from human or pig (Sus scrofa).

This module can be used in QuantSeq libraries prepared using the UMI Second Strand Synthesis Module for QuantSeq.

NOT Compatible with: QuantSeq FFPE and Pool, CORALL, LUTHOR HD and LUTHOR HD Pool.

BC1 Block Module for QuantSeq (Cat. No. 167)

Kits: QuantSeq FWD and REV kits only (Cat. No.191 - 196, 225).

Sample type: mouse brain/neuronal cell input RNA.

NOT Compatible with: QuantSeq FFPE and Pool, CORALL, LUTHOR HD and LUTHOR HD Pool.

Purification Module with Magnetic Beads (Cat. No. 022)

Purification protocols included in the following kits:

  • QuantSeq FWD, REV, FLEX and FFPE

  • CORALL

  • QuantSeq-Pool

  • Small RNA-Seq (optional: size selection)

  • LUTHOR HD

  • LUTHOR HD Pool

  • TeloPrime (post-PCR purification only! - please enquire via support@lexogen.com for details).

Gel Extraction Module (Cat. No. 054)

Kit: Small RNA-Seq Library Prep Kit.

TeloPrime PCR Add-on Kit V2 (Cat. No. 018)

Kit: TeloPrime Full-Length cDNA Amplification Kit V2.

Protocols: qPCR assay, endpoint PCR.

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