How are my CORALL data analyzed with Kangooroo?

The CORALL data analysis pipeline includes:

• UMI extraction

• Trimming (Cutadapt V1.18)

• FastQC analysis of trimmed FASTQ files (FASTQC V0.11.7)

• Alignment (STAR V2.6.1a)

• UMI collapsing (more info can be found here)

• Mix² for transcript isoform quantification (Mix² is a Lexogen´s proprietary software, for more information please visit our webpage Mix² RNA-Seq Data Analysis Software)

• Gene counting (FeatureCounts V1.6.4)

• Differential gene expression analysis (DESeq2 V1.18.1)