My FASTQ files exceed the size limit, what should I do?

The free data analysis runs included with our QuantSeq FWD/REV, QuantSeq FFPE and LUTHOR HD and CORALL library prep kits are limited to FASTQ files with a maximum size of 1.5, 3 and 10 GB, respectively.

For more information on the quotas included in the voucher code, please see this FAQ: Is there a size limit for the FASTQ file?

If you have larger files you have two options:

Option 1

You can compress your FASTQ files and/or sub-sample the reads to reduce the size of your FASTQ files.

To compress the files:

• For windows computers download 7-zip and re-zip with quality best.

• For Mac OS and Linux this can be done with the command line command:

gunzip -c some_file.fastq.gz | gzip -9 > some_higher_compressed_file.fastq.gz

To sub-sample reads:

• Check out this Subsampling a fastq file with awk a command line tutorial.

Option 2

You can purchase additional voucher codes for larger file sizes by contacting sales@lexogen.com.

Here are the catalog numbers you need: