Are there recommendations for low RNA input and serum/plasma samples?

Total RNA input amounts lower than 100 ng, small RNA-enriched smaples, and serum/plasma samples may require protocol adjustments. These adjustments include dilution of the 3' Adapter (A3), 5' Adapter (A5) and Reverse Transcription Primer (RTP) and adjusted endpoint PCR cycle numbers. Please see Appendix B of the User Guide.

The minimum amount of total RNA input depends on the small RNA content of the sample in question.

Increase the input material for total RNA samples with lower small RNA content.