Skip to main content
Skip table of contents

What are the input RNA requirements ?

1ng - 500ng of total RNA can be used as input for the miRVEL Discovery Small RNA-Seq Library Prep Kit. The recommended starting input is 100 ng of total high-quality RNA. Should you decide to work with small-enriched RNA samples, it is important to note that small RNA accounts for approximately 10% of the total RNA. For instance, from 100 ng of total RNA, you can expect to extract about 10 ng of small RNA. Therefore, it is recommended to use the adapters and RT primer dilutions designed for 100 ng of total RNA. For more information about protocol adjustments, please consult Appendix B of the miRVEL Discovery Small RNA-Seq Library Prep Kit User Guide.

JavaScript errors detected

Please note, these errors can depend on your browser setup.

If this problem persists, please contact our support.