The UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) is compatible with the following library prep kits:
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QuantSeq 3’ mRNA-Seq V2 Library Prep Kit FWD with UDI (Cat. No. 191 - 196)
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QuantSeq 3’ mRNA-Seq V2 Library Prep Kit FWD with UDI when used in combination with the QuantSeq-Flex First Strand Synthesis Module for Illumina V2 (Cat. No. 166)
These modules can also be used for QuantSeq library preparation where UMIs are included:
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Globin Block Modules for QuantSeq (Human and Pig, Cat. No. 070, 071)
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Lexogen UDI 12 nt Unique Dual Indexing V2 Add-on Kits (Cat. No. 198 - 203)
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UDI 12 nt Unique Dual Indexing Sets (Cat. No. 101 - 105, and 156)
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PCR Add-on Kit for Illumina (Cat. No. 208)
This UMI module is not compatible with the following kits:
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QuantSeq 3' mRNA-Seq Library Prep Kit REV for Illumina (Cat. No. 225)
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QuantSeq FFPE 3' mRNA-Seq Library Prep Kit FWD with UMIs (Cat. No. 222-223)
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QuantSeq-Flex Second Strand Synthesis Module for Illumina (Cat. No. 028)
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LUTHOR HD 3' mRNA-Seq Library Prep Kit for Illumina (Cat. No.204, 221)
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LUTHOR HD Pool ' mRNA-Seq Library Prep Kit for Illumina (Cat. No. 205)
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CORALL V2 Library Prep Kits (Cat. No. 177 - 182 for CORALL mRNA-Seq V2, Cat. No. 171 – 176, 183 - 186 for CORALL V2 Total RNA-Seq and Cat. No. 219-220, 233-234 for CORALL FFPE Total RNA-Seq)
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Small RNA-Seq Library Prep Kit for Illumina (Cat. No. 052, 058, 135)
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miRVEL Profiling and Discovery Small RNA-Seq Library Prep Kits (Cat. No. 242-243)
ATTENTION! The UMI Second Strand Synthesis Module for QuantSeq is also not compatible with, and not recommended for use with library prep protocols or kits from any other vendors.