How many reads per sample do I need ?
A general recommendation would be to have 5 - 10 million reads per sample.
For low input RNA amounts or libraries with lower small RNA content and depending on the downstream application, a higher number of reads per sample may be required.
Small RNA / miRNA Sequencing | Number of reads |
|---|---|
Differential Expression (Profiling) | 1 - 5 M |
Discovery | 5 - 10 M |
Table | Sequencing Read Recommendations by Application (Campbell et al., 2015).