What is the optimal sequencing read length for Small RNA-Seq libraries?

For Small RNA-Seq libraries, short read lengths are sufficient.

• For miRVEL Discovery, we recommend a 72 bp single-read protocol with 10 bp dual indexing. Alternatively, a 50 bp single-read protocol is adequate if unique molecular identifiers (UMIs) are not required.

• For miRVEL Profiling, a 50 bp single-read sequencing protocol with 8 bp dual indices is sufficient (miRVEL Profiling does not include UMIs).