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Is any manual intervention required during the run?

Yes. If thermocycler incubations are run off-deck, five such interventions (including PCR) are required. The machine stops and asks the operator to do various steps such as, take the plate with samples, seal it with film, perform the incubation step(s) on the external thermocycler, unseal the plate, place it on deck again, and resume the run.

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